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Abstract
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Background: Polygonum cuspidatum, a plant commonly used in traditional medicine, possesses various pharmacological properties, including anti-inflammatory and antioxidant properties. How- ever, understanding whether P. cuspidatum exerts anti-inflammatory effects and elucidating its underlying mechanisms remain limited. In this study, we evaluated the anti-inflammatory effects of P. cuspidatum hot water extract (PWE) using lipopolysaccharide (LPS)-induced RAW 264.7 cells and a λ-carrageenan (λCAR)-induced paw edema C57BL/6 mouse model.
Methods and Results: To evaluate the anti-inflammatory efficacy of PWE, a nitric oxide (NO) assay, quantitative reverse tranxxxscription polymerase chain reaction, and western blotting were per- formed. At PWE 400 ㎍/㎖, NO generation was reduced by 51.53 ± 1.94%, compared to that using LPS-stimulated RAW 264.7 cells. PWE also inhibited the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in a dose-dependent manner at the protein and mRNA levels. Additionally, an in vivo experiment was conducted using a λCAR-induced paw edema mouse model. Cytokine analysis in the λCAR-induced paw edema mouse model revealed that it strongly inhibited pro- inflammatory factors.
Conclusions: PWE has strong anti-inflammatory effects based on these results. We suggest that PWE can be used as a functional material for anti-inflammation and may be useful for preventing or treating inflammation.
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